Electronic tuning of self-healing fluorophores for live-cell and single-molecule imaging
نویسندگان
چکیده
Tri-Institutional Training Program in Chem Department of Physiology and Biophysics, W E-mail: [email protected] Department of Chemistry, Columbia Univer Departments of Psychiatry and Pharmac Physicians & Surgeons, USA Division of Molecular Therapeutics, New Yo † Electronic supplementary information ( Scheme S1, Tables S1 and S2, note on ca protective agents, methods of cellular im characterization of compounds. Supplem movies of living SNAPf-D2s-expressing C Cy5(4S)-AC(4) (right). Supplementary Mo living SNAPf-D2s-expressing CHO cells lab (right). See DOI: 10.1039/c6sc02976k Cite this: Chem. Sci., 2017, 8, 755
منابع مشابه
Electronic tuning of self-healing fluorophores for live-cell and single-molecule imaging† †Electronic supplementary information (ESI) available: Chart S1, Fig. S1 to S13, Scheme S1, Tables S1 and S2, note on calculating the effective concentration of protective agents, methods of cellular imaging and details the synthesis and characterization of compounds. Supplementary Movie 1: Single-molecule TIRF movies of living SNAPf-D2s-expressing CHO cells labeled with AF647 (left) or Cy5(4S)-AC(4) (right). Supplementary Movie 2: Single-molecule TIRF movies of living SNAPf-D2s-expressing CHO cells labeled with Dy549 (left) or Cy3(4S)-AC(4) (right). See DOI: 10.1039/c6sc02976k Click here for additional data file. Click here for additional data file. Click here for additional data file.
Bright, long-lasting organic fluorophores enable a broad range of imaging applications. "Self-healing" fluorophores, in which intra-molecularly linked protective agents quench photo-induced reactive species, exhibit both enhanced photostability and biological compatibility. However, the self-healing strategy has yet to achieve its predicted potential, particularly in the presence of ambient oxy...
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تاریخ انتشار 2016